Abstract
A high-performance liquid chromatographic (HPLC) method that uses UV—VIS detection and post-column derivatization with sodium hydroxide was developed for the determination of the growth-promoting antibiotic carbadox and three of its metabolites in swine muscle, liver and kidney tissues. Sample pre-treatment involves extraction with methanol—acetonitrile, purification over an alumina—Florisil column and partition with isooctane. A 2-ml volume of the final aqueous extract is injected into a column-switching HPLC system; detection is performed at 420 nm. The limits of determination are in the range 1–5 μg/kg. Preliminary experiments show a good precision with mean recoveries of 81–87% and a coefficient of variation of 4–10%. The method is highly selective and can be used in routine monitoring programmes.
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