Determination of Pydiflumetofen Residues in Rice and its Environment by an Optimized QuEChERS Method Coupled with HPLC-MS.

Abstract

Pydiflumetofen is a new succinate dehydrogenase inhibitor fungicide, and the method for determination of its residues in rice and associated environmental samples has not yet been reported. Here, we optimized, Quick Easy, Cheap, Effective, Rugged, Safe (QuEChERS) method for sample preparation, and used high performance liquid chromatography-tandem mass spectrometry (HPLC-MS) to detect the residual amounts of pydiflumetofen in rice and its environment. The results showed that there was a good linearity over the pydiflumetofen concentration range of 0.01-0.1mg/L in all matrices (R2 > 0.99). At the spiked levels of 0.01, 0.05, and 0.1mg/kg, the recovery rates of pydiflumetofen from various matrices were between 84.23 and 105.10 %, with the relative standard deviation of 1.07-9.99 %. The limit of detection (signal-to-noise ratio = 3) of the proposed method for pydiflumetofen was in the range of 1.9-3.5µg/kg, and the limit of quantification (signal-to-noise ratio = 10) was in the range of 6.3-11.7µg/kg.