Abstract
The synchronous fluorescence technique was developed for the determination of human serum albumin (HSA) in human body fluids with Thioguanine (6-TG) as a molecular probe. Under the optimal experimental conditions, the synchronous fluorescence peak of HSA–6-TG system is located at about 301nm and the enhancement synchronous intensity was proportion to the concentration of HSA. The calibration graphs are linear over the range of 0.69–552.0μgmL−1 with a correlation coefficient (R) of 0.9994. The detection limit was 0.133μgmL−1. It was successfully applied to determine the protein in human body fluids including serum, urine and saliva samples with 6-TG as probe with a satisfying result. In the analysis of human body liquids samples, the relative standard deviations (RSDs) were 0.55–3.31%, which obtained from 6 replicate determinations and recoveries were in the range of 97.0–104.1%.
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