Abstract
A sensitive high-performance liquid chromatographic (HPLC) method for the quantitation of the HMG-CoA reductase inhibitor pravastatin in human plasma is described. Sample preparation based on reversed-phase solid-phase extraction using triamcinolone acetonide as internal standard (I.S.). The compounds were separated on C 18 reversed-phase analytical column and then determined by ultraviolet detection. The recovery of pravastatin from plasma was 69.2±6.7% (mean±S.D.). The limit of detection for pravastatin in aqueous solution was 0.4 ng, the limit of quantitation in plasma was 2 ng/ml. In a preliminary pharmacokinetic study with two healthy volunteers the t 1/2 of pravastatin in plasma was found to be 0.8 and 2.3 h.
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