Abstract
Mitchell Petunia plants were analyzed for their level of ploidy by: (1) counting the number of chromosomes in root tips; (2) counting the number of chloroplasts per guard cell pair; and (3) using fluorescence microscopy to measure the amount of DNA stained with Hoechst 33258. The majority of plants propagated through tissue culture were chimeric rather than either completely diploid or haploid, and we have shown that one cannot distinguish between haploids and chimeric haploid/diploid plants based on chloroplast counts. One must use either chromosome counts or microfluorimetry to distinguish between these.
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