Abstract
Abstract Iran has more than 63 species of genus Onobrychis. This genus has small chromosomes with different ploidy levels. Counting ploidy levels in somatic cells in the metaphase is difficult; therefore, this study aims to evaluate a method using counts of stomata guard cell chloroplasts, as well as using flow cytometry as methods for determining ploidy levels in annual species of Onobrychis genus. Three middle leaves of greenhouse-grown plants were randomly selected for chloroplast counts in 10 pairs of stomata guard cells of the lower surface of leaf cells. For determining ploidy levels, the usual method was counting chromosomes which determined in the metaphase cells of root tip meristems with having minimum of 10 metaphase plate mitosis for each species. All of the samples were also studied, to determine ploidy levels using flow cytometry, and to find the mode and ratio for each of them. The results indicated that numbers of chloroplast stomata guard cells and flow cytometry in the tetraploid samples...
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