Abstract
BackgroundClerodendrum serratum Linn commonly known as Bharangi in India has wide applications in the Ayurveda and Siddha system of medicine which has been attributed to the treatment of various diseases like asthma, cough, fever, rheumatism, and cephalalgia ophthalmia. Squalene has nutritional, medicinal, and pharmaceutical health benefits, hence possess antioxidant and cytoprotective effects.MethodThe study presents the GC-MS analysis of phytoconstituents present in the Clerodendrum serratum roots and further estimation of one of the constituents, i.e., squalene which is ought to be present in the roots as per mass spectral data obtained. Squalene was determined from the ethanolic extract of C. serratum roots using GC-FID without derivatization.ResultsFour major constituents, i.e., squalene, methyl palmitate, hexadecenoic acid, and stigmasterol were detected by GC-MS. Squalene from the extract was eluted at 17.5min which was confirmed with the standard squalene peak eluted at the same retention time. The linearity range chosen was 5–30ug/mL, and the method was found to be pretty linear (R=0.995), accurate with satisfactory repeatability. Hence, the phytochemical compounds were detected by GC-MS and the squalene was determined and validated according to the ICH guidelines.ConclusionThus, the green gas chromatographic method can be used for quantification and qualification of active constituents in the roots of ethanolic extract of C. serratum. In addition, the presence of metabolite squalene by the GC-FID method was developed for the extract which is simple, fast, and environmentally friendly.
Highlights
Clerodendrum serratum Linn commonly known as Bharangi in India has wide applications in the Ayurveda and Siddha systems of medicine
Four major constituents, i.e., squalene, methyl palmitate, hexadecenoic acid, and stigmasterol were detected by Gas chromatography with mass spectrometry (GC-MS)
The phytochemical compounds were detected by gas chromatography (GC)-MS and the squalene was determined and validated according to the ICH guidelines
Summary
Clerodendrum serratum Linn commonly known as Bharangi in India has wide applications in the Ayurveda and Siddha systems of medicine. Several studies have confirmed that squalene showed nutritional, medicinal, and pharmaceutical health benefits (Das et al 2003) It has been extensively studied and has been reported to possess beneficial bioactivities including antioxidant, cytoprotective effects, and mainly as antitumor, suggested for supplementation which may be responsible for tumor growth inhibition and the prevention of normal cells from mutating into tumor cells when exposed to oxidative stress (Kohno et al 1995; Murakoshi et al 1992; Amarowicz 2009). Clerodendrum serratum Linn commonly known as Bharangi in India has wide applications in the Ayurveda and Siddha system of medicine which has been attributed to the treatment of various diseases like asthma, cough, fever, rheumatism, and cephalalgia ophthalmia. Medicinal, and pharmaceutical health benefits, possess antioxidant and cytoprotective effects
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