Abstract
Microdialysis is an important technology in the field of medicine, and with the popularization and development of microdialysis technology, it has been gradually applied to the field of ecology. In this study, microdialysis-HPLC as a new and environmentally friendly in situ technique was developed and applied for the first time to detect phenolic acid allelochemicals in Rehmannia glutinosa rhizosphere. A response surface analysis (RSM) was used to optimize the microdialysis in situ based on the Box–Behnken design (BBD) experiment. The optimum dialysis conditions were obtained as follows: the perfusion solution was a NaCl solution of 200 mmol/L with pH 4, and the dialysis temperature was 37 °C. Under these conditions, the content of phenolic acids in the rhizosphere 10, 20 and 30 cm away from the root was analyzed using high-performance liquid chromatography (HPLC) detection combined with the standard curve of phenolic acid addition. Compared to traditional methods, microdialysis-HPLC is a simple and environmentally friendly method for the in situ detection of phenolic acids in rhizosphere.
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