Abstract
Abstract A convenient and sensitive method based on the inhibition of the lipase-catalyzed hydrolysis by binding hydroxyl groups of lipase with organophosphorus pesticide is described for the enzymatic determination of organophosphorus pesticides. Employing the method proposed, the frequency response is proportional to organophosphorus pesticide concentration over the range of 250 ng/ml∼1.25 μg/ml. the detection limit is 58 ng/ml. The effects of temperature, pH value, incubation time and solvent are also investigated.
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