DETERMINATION OF OFLOXACIN IN OFLOXACIN EAR DROPS BY MICROFLUIDIC CHIP WITH CONTACTLESS CONDUCTIVITY DETECTION

Abstract

A method of microfluidic chip with contactless conductivity detection has been developed for the determination of ofloxacin in Ofloxacin Ear Drops. Electrophoresis conditions included a buffer of 1m mol L−1L- Histidine (pH 6.5) with 1m mol L−1 2-(N- Morpholino)ethanesulfonic acid (MES), an applied voltage of 1.5 kV, and an injection time of 10s with a voltage of 500V. Under optimum conditions, the component was determined within 0.7min, allowing detection of ofloxacin in Ofloxacin Ear Drops. The regression equation was y = 669767x– 1364.4 and the square of correlation coefficients (r2) was 0.9997. The linear range of the component was from 0.1 to 0.8 mg mL−1. The relative standard deviations (RSD, n = 3) for migration time and peak area were 0.9%and 4.0%, respectively. The detection limit (S/N = 3), 21.2 mg L−1, was low. The average recovery of the component was 101.7%(RSD = 4.6%, n = 3) by this method. This developed method is rapid, high efficient, sensitive, economical, and capable of the determining the active ingredient in Ofloxacin Ear Drops.