Determination of Nucleic Acids Sensitized by Emulsifier OP‐micelle Using Ethyl Rhodamine B as a Resonance Light‐Scattering Probe

Abstract

The resonance light scattering (RLS) spectra of ethyl rhodamine B with nucleic acid (calf thymus DNA and herring sperm DNA) have been studied. The effective factors and the optimum conditions have been studied, and the enhanced intensity of RLS is in proportion to the concentration of nucleic acids in the range 0∼5.00 µg mL−1 for calf thymus DNA (ctDNA) and 0∼3.50 µg mL−1 for herring sperm DNA (hsDNA). The limits of detection are 3.42 and 3.14 ng mL−1, respectively. Based on this, a RLS method for the determination of nucleic acids sensitized by emulsifier OP‐micelle was accordingly established. The binding mode concerning the interactions of ethyl rhodamine B with nucleic acids was also studied, and this method has good selectivity and high sensitivity and it has been applied to the determination of DNA in synthetic samples and real samples with satisfactory results.