Abstract
High-performance size exclusion chromatography on a TSK 3000 SW column was used to separate and quantify the individual milk proteins in their native and/or denatured state. Quantitation of the individual native whey proteins within ± 10% was achieved. The denatured proteins were dissociated into their monomer units by using sodium dodecyl sulphate and β-mercaptoethanol. A good separation of the whey protein monomers was achieved and the quantitation of the individual whey proteins within ± 10% was possible. Under these conditions, α s1- and β-casein were eluted as one peak, whereas κ-casein and ψ-casein were eluted in the same position as β-lactoglobulin and α-lactalbumin respectively. A method for calculating the areas and hence the concentration of the individual proteins in a mixture of casein and whey proteins is described.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.