Determination of morpholine residue in fruit and fruit juices by gas Chromatography−Tandem mass spectrometry

Abstract

A new analytical method involving gas chromatography-tandem mass spectrometry (GC−MS/MS) was developed for the determination of morpholine residues in fruit and fruit juice samples. The samples were extracted with acidic acetonitrile-water mixed solution, purified with a mixed cation exchange column, and subjected to gas chromatography-tandem mass spectrometry (GC–MS/MS). The isotope internal standard (morpholine-d8) method was used for quantification. The average recoveries ranged from 85.4 to 108.9%. The limit of quantification (LOQ) for all samples was 10.0 μg/kg. The positive samples were confirmed by gas chromatography-quadrupole-orbitrap high-resolution mass spectrometry (GC−Orbitrap HRMS). The developed method has good precision and accuracy.