Abstract
A method has been developed that uses a simplified sample preparation procedure and atomic absorption or electrochemical detection of HPLC eluents for the determination of methylmercury in fish. Methylmercury is isolated from the blended sample by chloroform elution from a diatomaceous earth-hydrochloric acid column. The organomercury compound is then extracted into a small volume of 0.01 N sodium thiosulphate solution. An aliquot of this solution is injected on to a Zorbax ODS column and eluted with methanol-ammonium acetate solution (3 + 2) buffer, pH 5.5 containing mercapto-ethanol. Detection can be accomplished by atomic-absorption spectrophotometry with the aid of a specially designed apparatus for the generation of mercury vapour. Alternatively, a commercially available electrochemical detector equipped with a dropping-mercury electrode may be used.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
