Abstract
A rapid and simple dispersive liquid−liquid microextraction method based on solidification of organic drop with high performance liquid chromatography-ultraviolet detection has been developed for the extraction and quantification of methocarbamol from human urine samples. The effect of extraction and disperser solvents (type and volume), sample solution pH, extraction time and salt effect was investigated. 1-Dodecanol and methanol were selected as extractive and disperser solvents, respectively. Placket-Burman experimental design and response surface methodology have been used for the method optimization. The linear dynamic range of 50–800 µg/L with a correlation coefficient of 0.9988 and a detection limit of 15 µg/L were achieved for the determination of methocarbamol under optimum conditions. The relative standard deviation was less than 3.2% (n = 6). After optimization, the method was successfully applied for the determination of methocarbamol in human urine samples.
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