Abstract
The aim of this study was to determine the difference between melatonin hormone in night milk and melatonin concentration in day milk of Holstein cows. In this study, daytime milk obtained from 40 heads Holstein cows in the first lactation raised in a private dairy cattle farm in Karapınar district of Konya state province in Turkey and night milk samples taken from the same cows that were kept in darkness for one week were used. The milk samples were collected from day and night milking in order to determine the melatonin concentration with the help of Bovine Melatonin (MLT) Elisa Kit. In this study, melatonin concentration in day time and night time milk were determined as respectively 103.70 ± 6.61 pg mL-1 and 163.13 ± 8.96 pg mL-1. The difference between melatonin levels of day and night milk was statistically significant (P<0.01). Since significant difference occurred between melatonin concentration between day and night milk at the end of the study, it can be stated that night milk can be used for medical purposes that is used cure of some illnesses and producers can be provided with a new production source.
Highlights
The aim of this study was to determine the difference between melatonin hormone in night milk and melatonin concentration in day milk of Holstein cows
Melatonin levels were determined with ELISA method from the samples of the daytime milk taken at the beginning of the study and the milk samples obtained only in the evening without any additional application from the same cows in the barn prevented from receiving light for a week
Melatonin level of the night milk in the dark is around 30 pg mL-1, while in the group with limited illumination it is between 15-20 pg mL-1
Summary
The aim of this study was to determine the difference between melatonin hormone in night milk and melatonin concentration in day milk of Holstein cows. The synthesis of melatonin is strictly controlled by lighting conditions and shows a clear circadian rhythm with low values during the daytime and significant increase at night (Karasek & Winczyk 2006; Srinivasan et al 2011) This hormone secret during sleep at night, is intensely secreted, especially between 23 and 05 hours. AA-NAT activity increases at the onset of darkness, which regulates the rate of melatonin synthesis It causes norepinephrine (NE) secretion to increase from the end of the postganglionic sympathetic nerve fibers present in the pineal gland. At the end of the experiment, the milk composition did not change and SCC decreased significantly in the middle of lactation in the implant group compared to the control group Because of these benefits of melatonin, determination of natural resources is very important. This study was conducted to determine the level of melatonin in milk obtained from day and night milking of Holstein cows, and the production status of a natural source of melatonin
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