Abstract
A quantitative hplc method for the simultaneous determination of lanatoside C and digoxin in Digitalis lanata was developed. The extract of dry leaf powder with 50% MeOH was applied to a Sep-PakC 18 cartridge prior to hplc analysis. The analysis was performed on an octylsilyl bonded silica column, using MeCN-MeOH-H 2 O (20:1:50) and uv detection (220 nm). The quantitation was carried out by the internal standard method. The proposed method is sufficiently precise and relatively simple. Application of this hplc analysis to the determination of lanatoside C and digoxin after fermentation of the leaf powder is also described
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