Determination of Ivabradine by Linear Sweep, Square Wave and Differential Pulse Voltammetric Methods Using Platinum Electrode in Pharmaceutical Preparation

Bilal Yilmaz,Bilge Akcay

doi:10.21608/aprh.2020.30205.1107

Abstract

Objectives: In this study, simple, fast and reliable cyclic voltammetry (CV), linear sweep voltammetry (LSV), square wave voltammetry (SWV) and differential pulse voltammetry (DPV) methods were developed and validated for determination of ivabradine in pharmaceutical preparations. Methods: The proposed methods were based on electrochemical oxidation of ivabradine at platinum electrode in acetonitrile solution containing 0.1 M LiCIO4.The well-defined oxidation peak was observed at 1.34 V. Results: The calibration curves were linear for ivabradine at the concentration range of 5-40 µg/mL for LSV, SWV and DPV methods, respectively. Intra- and inter-day precision values for ivabradine were less than 3.12, and accuracy (relative error) was better than 4.78%. The limits of detection values ranged from 1.20 to 1.60 µg/mL and quantification from 3.60 to 4.80 µg/mL. The mean recovery of ivabradine was 99.9% for pharmaceutical preparation. No interference was found from tablet excipients at the selected assay conditions. Conclusion: Developed methods in this study are accurate, precise and can be easily applied to Coralan tablet as pharmaceutical preparation.

Highlights

Ivabradine hydrochloride is 3-[3-[(7S)-3,4dimethoxy-7-bicyclo[4.2.0]octa-1,3,5-trienyl] methylmethylamino]propyl]-7,8-dimethoxy-2,5-dihydro-1H-3benzazepin-4-onehydro chloride (Figure 1)
This paper describes fully validated simple, rapid, selective and sensitive procedures for the determination of ivabradine employing linear sweep voltammetry (LSV), square wave voltammetry (SWV) and differential pulse voltammetry (DPV) methods the platinum disc electrode
Voltammetric behavior of ivabradine The electrochemical behavior of ivabradine was investigated at the Pt disc electrode in acetonitrile solution containing 0.1 M LiClO4 as the supporting electrolyte by using cyclic voltammetry (CV)

Summary

Introduction

Ivabradine hydrochloride is 3-[3-[(7S)-3,4dimethoxy-7-bicyclo[4.2.0]octa-1,3,5-trienyl] methylmethylamino]propyl]-7,8-dimethoxy-2,5-dihydro-1H-3benzazepin-4-onehydro chloride (Figure 1). It is a therapeutic agent used for the symptomatic treatment of chronic stable angina pectoris in patients with normal sinus rhythm who cannot take beta blockers. Several HPLC methods have been reported for the determination of ivabradine in urine, plasma and http://aprh.journals.ekb.eg/ 139. The reported methods were influenced by interference of endogenous substances and potential loss of drugs in the re-extraction procedure and involving lengthy, tedious and time-consuming plasma sample preparation and extraction processes and requiring a sophisticated and expensive instrumentation. The development of a new method capable of determining drug amount in pharmaceutical dosage forms is important. Redox properties of drugs can give insights into their metabolic fate or their in vivo redox processes or pharmacological activity

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Conclusion