Determination of inorganic ions and carbohydrates in cardioplegia and nephroplegia solutions by ion chromatography.

Abstract

In this study, methods for analyzing inorganic ions and carbohydrates in cardioplegia and nephroplegia solutions were developed and validated using ion chromatography with both conductivity and pulsed amperometric detection. The inorganic ions such as sodium, potassium, and calcium were separated by a cation-exchange column with 27mM methanesulfonic acid as mobile phase at 0.5mL/min. The anion (chloride) and carbohydrates (mannitol and glucose) were analyzed by an anion-exchange column using a mobile phase of 20mM sodium hydroxide at 1.0mL/min. The methods showed a high sensitivity for all analytes, with quantification limits from 0.0002 to 0.06mg/L. Good linearities between the peak areas and concentrations were found for all analytes within the selected concentration range (R2 >0.999). Relative standard deviation values for repeatability and interday precision were 0.1%-1.0% and 0.7%-1.6%, respectively. The accuracy was validated by determining the percentage recovery, which was between 98.0% and 101.3% for all analytes, indicating good accuracy of the methods. The robustness was verified by using an experimental design. Finally, real samples were analyzed to determine the content of the analytes. All assay values were between 96.8% and 102.5%.