Determination of imidacloprid and acetamiprid in bottled juice by a new DLLME-HPLC.

Abstract

A new kind of surfactant-emulsified vortex-assisted dispersive liquid-liquid microextraction method (SE-VA-DLLME) using benzyldimethyldodecylammonium chloride (BDDAC) as emulsifier and disperser has been developed for the determination of imidacloprid and acetamiprid in bottled grenadine and black currant juice samples prior to high-performance liquid chromatography-diode array detection. For grenadine juice and black currant juice, LODs were 0.78 and 0.45 μg/L and 0.81 and 0.83 μg/L and LOQs were 2.8 and 1.7 μg/L and 3.2 and 2.8 μg/L for imidacloprid and acetamiprid, respectively. The linear ranges were wider than 10-3000 μg/L with a correlation coefficient higher than 0.9913, the extraction recoveries were in the range of 61.6-84.2%, the enrichment factors were in the range of 27.0-43.3, and the recoveries and relative standard deviations of the studied neonicotinoids were in the range of 91.94-99.63% and 2.8-6.7%, respectively. The proposed method is presented as a simple, cheap, precise, accurate, and sensitive alternative for the determination of imidacloprid and acetamiprid in bottled grenadine juice and black currant juice samples.