Abstract
Hydrogen peroxide is determined by a chemiluminescence method with a reagent containing 100 μM luminol and 3 μM microperoxidase at pH 10 (carbonate buffer). Microperoxidase is superior to hematin as a catalyst. The method uses an automated flow injection system with a throughput of 2 samples per minute. The log—log calibration plot is linear (slope 1.3) from the detection limit, 3 × 10 -9 M up to 10 -5 M H 2O 2. The background emission is low. Impurities in the carrier stream and from some plastics may cause elevated background unless precautions are taken.
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