Abstract
4-Hydroxyproline, 8-hydroxyquinoline, 4-hydroxyquinoline-2-carboxylic acid and 4-hydroxyphenylacetic acid (HPA) react with H 2O 2 when irradiated with UV light to yield fluorescent products. Sub-micromolar detection limits of H 2O 2 are possible with HPA. The fluorescent product is the same as that formed in the peroxidase enzyme-mediated H 2O 2 oxidation of HPA. Several compounds that participate in the photomediated reaction do not react in the enzyme-mediated system. A mechanism for the photomediated reaction involving an aryloxy radical derived from the substrate is suggested. Although the limits of detection for H 2O 2 do not equal the best achievable with the enzyme-mediated systems, the simplicity of a single-step reaction and an ability to “photodevelop” the product offer a range of novel analytical possibilities.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
