Abstract
To determine the glutathione (GSH) content in a human hepatoma cell line (SMMC-7221) treated with xylitol/selenite, providing a part of an investigation of its anti-cancer mechanisms. The nuclei of SMMC-7221 cells were stained with Hoechst 33258 in an apoptosis assay, and their morphology subsequently changed from circular to crescent shape. The calibration curve (r(2)=0.992) was established, and GSH content markedly decreased after treated with 0.5 and 1mg xylitol/selenite l(-1) for 12, 36 and 60h (12h: from 95.57±19.57 to 29.09±7.74 and 24.27±11.15; 36h: from 70.73±11.35 to 19.54±6.39 and 9.35±6.69; 60h: from 72.63±16.94 to 7.432±3.84 and 0). The depletion rate of GSH was more related to the concentration of xylitol/selenite than the treatment time (from 69.95±1.87 to 100% vs. 0.22±0.2 to 100%). Xylitol/selenite is a promising anti-cancer drug to induce apoptosis in SMMC-7221 cells. It may regulate the apoptosis through the co-action of multiple mechanisms related to GSH depletion.
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