Abstract
An immunosensors/sequential injection analysis system is proposed for the assay of thyroid hormones free L-T4 and free L-T3 in blood samples. The working concentration ranges for free L-T4 and free L-T3, are between 2.0 and 720.0 pmol/L and between 2.0 and 340.0 pmol/L, respectively, with detection limits of 1.0 and 1.7 pmol/L, respectively. The selectivity of the immunosensors, as well as the recovery tests done for free L-T4 and free L-T3 (99.90 ± 0.01% (n = 10) and 99.94 ± 0.02% (n = 10)), made the immunosensors/sequential injection analysis system suitable for the direct assay of thyroid hormones in blood samples.
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