Determination of Four Kinds of Sulfonamides in Aquatic Products by Flow Injection On-line Preconcentration and High Performance Liquid Chromatography

Abstract

Using activated carbon fibre as the absorbent,a flow injection on-line preconcentration-high performance liquld chromatographic method for the determination of four kinds of sulfonamides(sulfathiazole,sulfamethazine,sulfamonomethoxine and sulfaquinoxaline) in aquatic products was established.The samples were extracted by phosphate buffer solution,then preconcentrated on activated carbon fibre by flow injection.The separation was carried out with an Eclipse XDB-C18 column(250 mm×4.6 mm,5 μm) in the mobile phase system of formic acid solution-formic acid methanol solution(60 ∶ 40,V/V) at pH=3.0,the flow rate was 1.0 mL/min.The detection was performed at 270 nm wavelength.The experiment result showed that the good linear relationship was obtained in the sulfonamides concentrations from 0.001-0.064 mg/L with the correlation coefficient from 0.9997 to 0.9999.The detection limit was 0.48-1.15 μg/L(S/N=3).The enrichment factors for the four sulfonamides were from 137 to 406.The recoveries were 93.5%-104.0%.This method was easy to operate with precise result and good repetition and can be successfully applied to the determination of sulfathiazole,sulfamethazine,sulfamonomethoxine and sulfaquinoxaline.