Determination of Four Endogenous Phytohormones in Bamboo Shoots by Liquid Chromatography-Tandem Mass Spectrometry

Abstract

A method was developed for the determination of four endogenous phytohormones,gibberellins,3-indolebutytic acid,abscisic acid and indole-3-acetic acid in bamboo shoots by liquid chromatography-tandem mass spectrometry( LC-MS / MS). The four analytes were extracted from bamboo shoots by methanol-formic acid( 99 ∶ 1,V / V),and purified by solid extraction using C18sorbent,and then analyzed by liquid chromatography-tandem mass spectrometry( LC-MS / MS). The four analytes were then separated on a reversed phase column with mobile phase containing water and methanol in a gradient elution mode. Qualitative analysis was performed using multiple-reaction monitoring( MRM). Matrix-matched calibrations were respectively used to quantify the analytes concentrations. The recoveries were in the range of 75. 2%-105. 0%,and the relative standard deviations( RSDs) were 0. 8%-11. 4% at three spiked levels of 5,50 and 200 μg / kg for all analytes. The limits of quantification( LOQ) for all analytes were between 0. 1 and 1. 0 μg / kg.