Determination of Extractive Value, Phytochemical Constituents and In-Vitro Anti-Urolithiatic Activity of Embelia ribes Burm. F. and Ipomea Hederacea Jacq.

Abstract

Objective: The study was planned to investigate the extractive value and in-vitro anti-urolithiatic activity of different extracts of Embelia ribes Burn. F. and Ipomea hederacea Jacq seeds. Methods: Phytochemical analysis was undertaken to evaluate the phyto-constituents present in different extracts. The extracts were prepared by the maceration process. The in-vitro anti-urolithiatic activity was performed by dissolution and turbidity methods. Result: The extractive value of chloroform, ethanol, diethyl ether, n-hexane, acetone and water of Embelia ribes and Ipomea hederacea was found to be 2%, 2.5%, 2.5%, 2%, 1.5%, 1%w/w and 1.5%, 2%, 2.4%, 14%, 2%, 6% w/w respectively. The result of phytochemical analysis showed that aqueous, ethanolic, chloroform, diethyl ether, n-hexane and acetone extract of Embelia ribes and Ipomea hederacea have positive for alkaloids, proteins, carbohydrates, amino acids, tannins, protein, mucilage and glycoside. Moreover the result of anti-urolithiatic activity of ethanol and hexane extract of Embelia ribes and Ipomea hederacea were found to be 84.33±0.67%, 76.67±0.67%, 72.33±0.88 NTU and 73.00±1.15 NTU by dissolution and turbidity methods respectively. Conclusion: On the basis of result analysis, we can conclude that both the plants extract have anti-urolithiatic activity but ethanol and hexane extract have much significant activity than other extracts when compared to standard cystone.