Abstract
A determination method for estriol 3-sulfate 16-glucuronide (E3-3S-16G) in pregnancy serum by liquid chromatography/electrospray ionization-tandem mass spectrometry has been developed. E3-3S-16G gave an intense deprotonated ion in the negative-ion mode, which provided a product ion formed from the elimination of the glucuronic acid moiety by the collision induced dissociation. The serum specimen was diluted, heated to remove protein followed by a solid phase extraction using an OASIS HLB cartridge, then subjected to liquid chromatography/tandem mass spectrometry. The recovery of E3-3S-16G from serum was satisfactory and there was no interfering peak derived from the serum components at the elution position of E3-3S-16G, but the ionization efficiency of the analyte was influenced by some substances from the serum and varied among each specimen. To overcome this problem, the standard addition method was employed in the present study and the inter-assay coefficient of variation was acceptable. The developed method was applied to serum samples obtained from pregnant women in their third trimester and gave satisfactory results.
Published Version
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