Abstract
A new method for the fluorometric detection of K 1 and K 2 vitamins using post-column electrochemical reduction is described. Dual electrochemical detection in the reduction/reoxidation mode for coulometric/coulometric as well as coulometric/amperometric detection appears to be more sensitive and selective towards the plasma background than simple reductive electrochemical detection, but fluorometric detection after coulometric reduction offers the best results. Combination of normal-phase chromatography and the described method is only possible if supporting electrolyte is added post-column, but leads to higher detection limits. This highly sensitive method is applied to the determination of vitamin K 1 in human plasma samples.
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