Abstract
A method which combines UV spectroscopy, guest–host chemistry and principal component regression (PCR) was proposed for determining the enantiomeric composition of DOPA samples. The calibration models were developed from UV spectral data of a series of samples containing DOPA with different known enantiomeric compositions by using PCR. The obtained model was subsequently validated by determining the enantiomeric composition of a set of independently prepared samples. This method shows high sensitivity for determining the enantiomeric composition of DOPA. When there is 5.00 μM DOPA in the samples, the enantiomeric composition of DOPA can be accurately determined.
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