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Abstract
Abstract A rapid gas-liquid chromatographic (GLC) procedure has been developed for the determination of docosenoic acids in fats and oils. The method involves conversion of a known weight of oil to methyl esters using sodium methoxide-methanol, with a known weight of methyl tetracosanoate used as an internal standard. After acidification and extraction into hexane, esters are analyzed by GLC on a diethylene glycol succinate column. The percentage of docosenoic acids is calculated from the docosenoate and internal standard peak areas. The method is particularly suited to the determination of low levels (<5%) of docosenoic acids.
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