Abstract
Determination of Dissociation Constants of Coenzymes and Abortive Ternary Complexes with Rabbit Muscle Lactate Dehydrogenase from Fluorescence Measurements
Highlights
Muter&&---Lactate dehydrogenase was a product of Boehringer and Sons and the sodium salt of NADPH was purchased from Sigma Chemical Company
The question necessarily arises in studies of the type presented in this report as to whether one is measuring dissociation constants associated with enzymatic activity
It seems quite unlikely that values for four enzyme-substrate dissociation complexes could, from kinetic and fluorometric experiments, agree so well for lactate dehydrogenase, by chance
Summary
The purpose of the present report is to present data obtained from fluorescence experiments of rabbit muscle lactate dehydrogenase
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Published Version
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