Abstract
The aim of the study was to develop an algorithm for directed analysis of diphenhydramine in biological extracts from urine and blood using a unified method of HPLC research.Materials and methods. The extraction of diphenhydramine was carried out with chloroform at pH 9.0. The extracts were purified from impurities by a combination of TLC and extraction with hexane. TLC purification and identification of diphenhydramine were carried out under optimal conditions: organic solvents systems – chloroform-methanol (90:10); methanol; methanol-25 % solution of ammonium hydroxide (100:1.5) and chromatographic plates - Sorbfil PTLC-AF-A, Sorbfil PTLC-P-B-UV. For the detection of diphenhydramine, the most sensitive location reagents were used - UV light (λ = 254 nm) and Dragendorff reagent in the modification of Mounier.HPLC analysis was carried out on a microcolumn liquid chromatograph Milichrome A-02 in conditions: reversed-phase variant, column with non-polar sorbent Prontosil 120-5 C18 AQ, 5 μm; mobile phase in the mode of linear gradient – from eluent А (5 % acetonitrile and 95 % buffer solution) to eluent B (100 % acetonitrile) as during 40 min. The flow rate of the mobile phase has been formed 100 μl/min, injection volume – 4 μl. Multichannel detection of the substance was carried out using a UV spectrophotometer at 210, 220, 230, 240, 250, 260, 280 and 300 nm; the optimal value of column temperature – 37 - 40°С and pressure of pump – 2.8 – 3.2 MPa.Results and its discussion. Extraction, purification, identification and quantitative determination of diphenhydramine were carried out according to the developed methods. It is established that when isolating diphenhydramine from blood according to the developed methods it is possible to allocate 34.2 – 38.4 % of substance ( = ± 5.69 %, RSD = 2.04 %) and from urine – 55.8 – 60.5 % of substance ( = ± 3.91 %, RSD = 1.40 % ).Conclusions. An algorithm has been developed for directed analysis of diphenhydramine in biological extracts from urine and blood using a unified HPLC method. Statistical processing of the experimental results indicates the reliability and reproducibility of the technique
Highlights
Diphenhydramine hydrochloride – 2diphenylmethoxy-N,N-dimethylethylamine hydrochloride – first generation of H1-histamine receptor blocker
The aim of the study was to develop an algorithm for directed analysis of diphenhydramine in biological extracts from urine and blood using a unified method of high performance liquid chromatography (HPLC) research
The concentration of diphenhydramine in methanol solution (C, μg/ml), which was obtained after purification of biogenic extracts by TLC and extraction methods, was calculated using a calibration graph or the equation of the line corresponding to the calibration graph
Summary
Diphenhydramine hydrochloride (dimedrol) – 2diphenylmethoxy-N,N-dimethylethylamine hydrochloride – first generation of H1-histamine receptor blocker. The effect on the central nervous system occurs due to the blockade of H3-histamine receptors in the brain and inhibition of the central cholinergic structures. The drug has a pronounced antihistamine activity, reduces or prevents histamine-induced smooth muscle spasms, increasing capillary permeability, tissue edema, itching and hyperemia. Diphenhydramine causes the effect of local anesthesia, blocks the cholinergic receptors of the ganglia, lowers blood pressure, has sedative, hypnotic, antiparkinsonian and antiemetic effects. According to Li Y.Y. and co-authors diphenhydramine in the prophylactic process reduces bladder discomfort in patients in the postoperative period [4]. Pickett L.E. and coauthors found that the use of diphenhydramine is effective for patients with an allergy to lidocaine during skin surgical procedures [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
