Abstract
The aim of this work is identification and quantification of clonidine hydrochloride by a unified HPLC method, which allows to obtain reliable and reproducible research results.Materials and methods. HPLC analysis was carried out on a microcolumn liquid chromatograph Milichrome A-02 in conditions: reversed-phase variant, column with non-polar sorbent Prontosil 120-5 C18 AQ, 5 μm; mobile phase in the mode of linear gradient – from eluent А (5 % acetonitrile and 95 % buffer solution) to eluent B (100 % acetonitrile) as during 40 min. The flow rate of the mobile phase has been formed 100 μl/min, injection volume – 4 μl. Multichannel detection of the substance was carried out using a UV detector at 210, 220, 230, 240, 250, 260, 280 and 300 nm; the optimal value of column temperature – 37 - 40°С and pressure of pump – 2.8 – 3.2 MPa.Results and its discussion. As a result of studies using a unified HPLC method, were obtained the retention parameters of clonidine hydrochloride and spectral relationships, which made it possible to include the results obtained in the database for the identification of antihypertensive drugs in the therapeutic monitoring of treatment with an individual drug, or comprehensive treatment of diseases of the cardiovascular system. The development of the quantitative determination of clonidine hydrochloride by HPLC on model solutions using various concentrations of the drug was carried out. The content of clonidine hydrochloride was determined according to the equation S = 0.5 · 10-4 C + 1.8 · 10-3; the correlation coefficient was 0.9964. It is established that the relative uncertainty of the average result did not exceed +2.12 % when HPLC analysing of clonidine hydrochloride in model solutions.Conclusions. Identification and quantification of clonidine hydrochloride by a unified HPLC method, which allows to obtain reliable and reproducible research results were conducted. The results of research by a unified HPLC method can be recommended for implementation in the practice of forensic bureaus, toxicological centers, clinical laboratories for the study of drugs in biological objects
Highlights
Clonidine hydrochloride – N–(2,6-dichlorophenyl)4,5-dihydro-1H-imidazol-2-amine hydrochloride is the antihypertensive drug
The suitability of the chromatographic system for high performance liquid chromatography (HPLC) studies of clonidine hydrochloride was confirmed in determining the coefficients of the symmetry of the peaks of the substance (Ks); coefficients of capacity ratio (k')
Retention parameters of clonidine hydrochloride obtained as the result of HPLC analysis (n=5, Р=95 %)
Summary
Clonidine hydrochloride – N–(2,6-dichlorophenyl)4,5-dihydro-1H-imidazol-2-amine hydrochloride is the antihypertensive drug. Clonidine is used to treat hypertensive crisis, arterial hypertension. The hypotensive effect of clonidine is accompanied by a decrease in cardiac output and a decrease in the peripheral resistance of blood vessels, including blood vessels of the kidneys. Due to its effect on the central nervous system, clonidine has an analgesic and sedative effects. G. and co-authors were conducted studies that confirmed the efficacy and safety of clonidine as a sedative in critically ill patients with artificial lung ventilation [3]. C. and co-authors were proved the effectiveness of the use of clonidine as a sedative in children with artificial lung ventilation [4]. Clonidine is used to treat withdrawal symptoms in opioid addiction. J. and co-authors were obtained positive results with daily clonidine intake as an adjunct to buprenorphine therapy for opioid dependence [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
