Determination of Di(2-ethylhexyl phthalate) released in the blood from PVC line during the hemodialysis procedure

Beatriz Luci Fernandes,Lia Sumie Nakao,Max Ingberman,Fernanda Maria,Marcia Regina Cubas

doi:10.1590/s1517-707620170001.0282

Abstract

ABSTRACT The objective of the work is to demonstrate the applicability of the High-Performance Liquid Chromatography (HPLC) on the quantification of the Di(2-ethylhexyl phthalate) (DEHP) released from the Polyvinyl Chloride (PVC) line into the circulating blood during the hemodialysis procedure. As the proposal is not to validate the method, blood samples from five patients and three healthy volunteers were collected, and the plasmas were isolated, prepared and analyzed. The average concentration of DEHP in the patient's plasma was 0.19±0.12 mg/kg of body mass, while in the plasma of the healthy volunteers was 0.003±0.002 µg/kg of body mass. The results showed that the method presented is an alternative to quantify the DEHP in the blood and could assist in the search for alternative materials to apply to extracorporeal circulation equipment. The study was performed according to the national and international standards of ethics in research involving human beings (CEP PUCPR Prot. n.5802).

Highlights

The Chronic Kidney Disease (CKD) is characterized by a gradual loss of kidney function resulting in increasing of the metabolites concentration in plasma from the protein metabolism
Take into account the importance to quantify the Di(2-ethylhexyl phthalate) (DEHP) in the blood to evaluate its systemic effects, the aim of this study is to present a clear procedure to prepare blood samples for High-Performance Liquid Chromatography (HPLC) to quantify the DEHP released in the blood of patients during hemodialysis procedures
Table 2 shows the results of the linear regression of the curves generated during the HPLC analysis for the volunteer's patients (P) and the healthy volunteers (V)

Summary

Introduction

The Chronic Kidney Disease (CKD) is characterized by a gradual loss of kidney function resulting in increasing of the metabolites concentration in plasma from the protein metabolism. Such a situation causes an increase in urea and creatinine that obligates the patient to undergo long treatment, often leading to chronic complications [1]. Hemodialysis therapy is carried out through the decontamination of blood during extracorporeal circulation. Reuse is a normal practice in some hemodialysis centers, it is a controversial subject for many researchers and health professionals, since some studies have shown that reuse has increased the number of hospitalization and mortality [5] [6] [7]

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Conclusion