Abstract
A sensitive method by fluorescence quenching for the determination of dexamethasone sodium phosphate is proposed. The method is based on the ability of phosphate to inhibit the formation of a strong fluorescent complex of Tb3+ with tiron. The fluorescence intensity trends equilibrium after a 20-minutes reaction of dexamethasone sodium phosphate and Tb3+-tiron complex. The optimal conditions for the determination of dexamethasone sodium phosphate are studied. Under optimal conditions, the linear range is 2.5 × 10−7–4 × 10−6 mol/L and the detection limit is 6.2 × 10−8 mol/L, and the relative size distribution (RSD) of 11 times determination are 2.2% and 0.8% for 5 × 10−7 mol/L and 1 × 10−6 mol/L, respectively. The developed method is successfully applied to assay the dexamethasone sodium phosphate content in pharmaceutical injections.
Published Version
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