Abstract
Background: Clopidogrel, is a thienopyridine derivative labeled for use to prevent thrombosis after coronary artery stenting. Pharmacokinetics of clopidogrel is studied indirectly by quantification of carboxylic acid which is a major metabolite of Clopidogrel. Objective: The aim of this work is to develop and validate a rapid, simple and sensitive LC/MS/MS assay method for the determination of Clopidogrel carboxylic acid in human plasma using Clopidogrel-D4-carboxylic acid as internal standard. Methods: Analytes was extracted from 200 μl of plasma by a simple liquid-liquid extraction using diethyl ether – n-hexane (80:20, v/v). The chromatographic separations were achieved on a C18 column using Methanol, de-ionized water and formic acid as a mobile phase at flow rate of 0.5 ml/minute. Analysis was monitored by multiple reactions monitoring mode based on m/z transition of 308.10→113 for Clopidogrel carboxylic acid and 312.10→129 for internal standard. Result: The method had a total run time of about 4 minutes. The lower limit of quantification (LLOQ) was 25 ng/ml showing good linearity over the working range of 25 – 3000 ng/ml (r ≥ 0.999). The intra- and inter day accuracies were 90% - 98% and 92.138% - 96.889% respectively (deviation within acceptable range ≤ 10%).Conclusion: It was shown that this method is suitable for pharmacokinetic study following oral administration of Clopidogrel and can be successfully applied to the therapeutic drug monitoring of Clopidogrel in Clopidogrel-treated patients.
Highlights
Clopidogrel hydrogen sulfate (Figure 1), methyl (+)-(S)α-(o-chlorophenyl)-6,7-dihydrothieno[3,2-c]pyridin-5(4 H)-acetate hydrogen sulfate, is a novel thienopyridine derivative that irreversibly blocks adenosine diphosphate (ADP) and is important in platelet aggregation, the cross-linking of platelets by fibrin
The intra- and inter day accuracies were 90% - 98% and 92.138% - 96.889% respectively.Conclusion: It was shown that this method is suitable for pharmacokinetic study following oral administration of Clopidogrel and can be successfully applied to the therapeutic drug monitoring of Clopidogrel in Clopidogrel-treated patients
The present study report a simple and sensitive liquid chromatography coupled to tandem mass spectrometric method (LC-MS/MS) for determination of Clopidogrel carboxylic acid in human plasma using Clopidogrel-d4-carboxylic acid as the internal standard
Summary
Clopidogrel hydrogen sulfate (Figure 1), methyl (+)-(S)α-(o-chlorophenyl)-6,7-dihydrothieno[3,2-c]pyridin-5(4 H)-acetate hydrogen sulfate, is a novel thienopyridine derivative that irreversibly blocks adenosine diphosphate (ADP) and is important in platelet aggregation, the cross-linking of platelets by fibrin. Studies showed that the active compound clopidogrel is the S-enantiomer [5,6]. The major circulating compound, is an inactive carboxylic derivative, which is used to document the pharmacokinetic profile of clopidogrel [8]. Pharmacokinetics of clopidogrel is studied indirectly by quantification of carboxylic acid which is a major metabolite of Clopidogrel. Objective: The aim of this work is to develop and validate a rapid, simple and sensitive LC/MS/MS assay method for the determination of Clopidogrel carboxylic acid in human plasma using Clopidogrel-D4-carboxylic acid as internal standard. The intra- and inter day accuracies were 90% - 98% and 92.138% - 96.889% respectively (deviation within acceptable range ≤ 10%).Conclusion: It was shown that this method is suitable for pharmacokinetic study following oral administration of Clopidogrel and can be successfully applied to the therapeutic drug monitoring of Clopidogrel in Clopidogrel-treated patients
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