Determination of Ceftizoxime in Human Abscess Fluid by Paired Ion Reversed-Phase HPLC

Abstract

Abstract An ion paired reversed - phase high performance liquid chromatographic assay for ceftizoxime in human abscess fluid using barbital as the internal standard is described. Sample preparation consists of precipitating the proteins in diluted abscess fluid with methanol, and centrifuging. Decanted supernatant after evaporation to dryness is reconstituted with the HPLC mobile phase for injection. The HPLC separation was carried out on an octyl C8 column using a mobile phase composed of potassium phosphate buffer, tetrabutylammonium dihydrogen phosphate IP reagent and 20% methanol (v/v). UV detection was at 254 nm and the concentration range for the assay was 2.0μg/mL to 50.0μg/mL. The results of the full validation of the assay gave coefficients of variation for inter-assay variability of 8.4–12.6% and intra-assay variability of 4.3–9.3% for three levels of concentration. This isocratic HPLC method is relatively simple, rapid and shows good reproducibility for the determination of ceftizoxime in human...