Abstract
A simple, rapid and sensitive method for the clean-up and analysis of cefoxitin in serum and tissue is described. Serum (0.5 ml) and tissue (100 mg) samples after homogenization underwent high speed centrifugation. Chromatography was performed on a μBondapak C 18 cartridge using a mobile phase of 0.005 M potassium dihydrogen phosphate–acetonitrile–glacial acetic acid (77.5:22:0.5, v/v/v) with a flow-rate of 2.0 ml/min. Ultraviolet detection occurred at 235 nm. The procedure produced a linear curve for the concentration range 100–5000 ng/ml. The assay produced accurate, repeatable and rapid results for both tissue and serum samples without the need for chemcial extraction.
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Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
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