Determination of Catechol by a Laccase Biosensor Based on Silica-Modified Zirconia Nanoparticles

Abstract

A promising nanotechnological material, zirconia nanoparticles modified with SiO2, was used as a medium for the immobilization of laccase to construct a novel biosensor that exhibits sensitive amperometric response to catechol in 0.1 mol · L−1 phosphate buffer (pH 6.0) using cyclic voltammetry. The linear response to catechol was from 1.0 × 10−6 to 1.0 × 10−4 mol · L−1 and the detection limit was 3.5 × 10−7 mol · L−1 at a signal-to-noise ratio of 3. The biosensor exhibited good stability, precision, and few interferences.