Determination of carotenoids in flowers and food supplements by HPLC-DAD

Petra Pavelková,Věra Schulzová,Aleš Krmela

doi:10.2478/acs-2020-0002

Abstract

AbstractMarigold flowers (Tagetes patulaandCalendula officinalis) were chosen for analysis because they are the most often used source of lutein and its isomer zeaxanthin for the production of food supplements on the Czech market. Direct extraction and extraction with alkaline hydrolysis were compared to detect free or bound carotenoids. For carotenoid separation, C18 and C30 columns were used. A new method for determination of carotenoid content in food supplements in form of capsules has been developed and validated. All matrices were analysed by high-performance liquid chromatography with diode array detection (HPLC-DAD). It has been found that alkaline hydrolysis is required for both Marigold flowers and food supplements to release lutein from ester bonds to fatty acids. InCalendula officinalislutein in the concentration of 807—1472 mg·kg−1of dry matter was detected.Tagetes patulahas been identified as a better lutein source with the content of 5906—8677 mg·kg−1of dry matter. It has been found that the content of lutein and zeaxanthin in commercial food supplements (Lutein Complex PremiumandOccutein Brillant) is consistent with the declared quantity. Linearity of the HPLC-DAD method ranged from 0.1—20 μg·mL−1with the limit of quantification (LOQ) of 1.7 mg·kg−1for lutein in Marigold flowers and 200 mg·kg−1in food supplements. Repeatability was 2.3 % for lutein in all tested matrices.

Highlights

Carotenoids are ubiquitous organic compounds, mainly of yellow, orange and red colour, soluble in fats and organic solvents (Watkins and Pogson, 2020)
The performed experiments aimed to determine the content of free and bound lutein/zeaxanthin in matrices used for the production of food supplements (Calendula officinalis, Tagetes patula) and the content of these xanthophylls in food supplements as well as to compare the determined levels with the producer’s declaration
C18 column was sufficient for the determination of total lutein and zeaxanthin content as the time needed for separation is significantly shorter, approximately twice, compared to the C30 column separation

Summary

Introduction

Carotenoids are ubiquitous organic compounds, mainly of yellow, orange and red colour, soluble in fats and organic solvents (Watkins and Pogson, 2020). More than 1100 carotenoids are known (Yabuzaki, 2017). Several of these pigments are retinoids because of their vitamin A activity (Becerra et al, 2020; Watkins and Pogson, 2020). From the chemical point of view, these compounds are predominantly tetraterpenes composed of eight isoprene units. In their chain, they have a conjugated double bond system representing a lightabsorbing chromophore that gives carotenoids their characteristic colour (Rodriguez-Amaya, 2019). Carotenoids are identified from their visible absorption spectrum (Saini et al, 2015)

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Conclusion