Abstract
A simple, direct method for the determination of total calcium in urine by manual microtitration with EDTA at pH 12, with Calocin as fluorescent indicator, is proposed. By use of urine sample volumes of only 0.01–0.05 ml, interference from phosphate and natural fluorescence is minimized. For strongly naturally fluorescing urines the addition of potassium dichromate provides effective quenching. Agreement between results obtained by the proposed method and by atomic-absorption spectroscopy for a series of urine samples was satisfactory.
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