Abstract
ABSTRACTThe use of nanomaterials to improve the sensitivity of enzyme-linked immunosorbent assays (ELISA) for small molecules allows wider application of this method. In this work, gold nanoflowers were synthesized by a seed-mediated procedure and used to prepare a horseradish peroxidase-immunoglobulin G-gold nanoflower complex. The complex was used as the signal amplification probe in a novel ELISA for bisphenol A. The gold nanoflower-enhanced ELISA demonstrated excellent detection sensitivity of 0.05 ng/mL, which was 20 times more sensitive than a standard ELISA procedure. A linear dynamic range from 0.1 to 5.0 ng/mL was obtained by this approach. The gold nanoflower-enhanced ELISA was used to determine bisphenol A in lake water, with recoveries from 92.0 to 102.0% in samples fortified at concentrations of 0.5, 1.0, and 3.0 ng/mL. The gold nanoflower-enhanced ELISA was shown to offer good sensitivity, simplicity, and low cost.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
