Abstract
Reversed-phase ion pair chromatography with UV detection was used to determine nine type VI and VIII bisbenzylisoquinoline alkaloids: aromoline, obamegine, berbamine, homoaromoline, colorflammine, thalrugosine, norobaberine, tetrandrine and isotetrandrine. The eluent was acetonitrile—0.05 M dihydrogen phosphate buffer (pH 3.0) (27:73, v/v) containing 0.1% diethylamine and 2 m M sodium heptanesulphonate. Five alkaloids were determined in the stem woods of a Lauraceous plant, Dehaasia triandra Merr.
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