Validated High-Performance Liquid Chromatography Method for Quantitative Determination of Anthracenderivatives in Decoction,Syrup and Water-Alcohol Extract of Frangula alnus Mill. Bark

Abstract

Nowadays, people pay much attention to the quality of herbal medicines. Because of the significant variation in active components in them, the quality control of herbal medicines is a very important issue. High-performance liquid chromatography (HPLC) is widely used in identification and quality control of herbal medicines. A new, simple, sensitive, selective, and precise high-performance liquid chromatography (HPLC) was developed for the determination and identification of frangulin A content in “Frangula syrup”, “Frangula decoction” and “solution of aqueous-alcoholic extract from the bark of Frangula alnus” was developed and validated. The stationary phase was inert sil C18 column. The mobile phase consisting of acetonitrile (HPLC Grade) and potassium dihydrogen phosphate buffer (pH 2.5) in a gradient flow was used. The column was equilibrated with the mobile phase (flow rate 1.0 ml/min); the UV detection was set at 420 nm. Various optimizations were performed to examine the frangulin A content in herbal medicines and their preparation including retention time, spike authentic standard, change of wavelength, change of mobile phase composition and blank test. Using HPLC analysis we obtained the results of the quantitative determination of content of frangulin A in decoction, syrup and water-alcohol extract of Frangula alnus Mill. bark. The content of frangulin A in decoction amounted to 6,45 µg/20 l, in syrup amounted to 3,72 µg/20 l, in water-alcohol extract amounted to 7,9 l /20 µg .