Abstract
Separation of Bicarbonate had been investi gated using a non-suppress cation chromatography and conductivity detection. Analysis was performed on an Optimix C18/SCX Cation-exchange column coupled to a conductivity detector, with an injection volume of 25 &muL and a flow rate of 0.2 mL/min. The leacheate was fermentation of cyanobacterial apart from sodium bicarbonate. Under the optimized conditions, the linear relationship of Bicarbonate was 0.99946 in the range between 01 and 500 mg/L. The relative standard derivations of Bicarbonate less than 1.0% (n = 8). The recoveries of Bicarbonate in the samples from 100.15% to 111.10% were obtained. The Limit Of Detection (LOD) was 0.5 mg/L. The result showed that the method was convenient and accurate for the determination of Bicarbonate in Cyanobacterial fermentation.
Highlights
There are four forms of Dissolved Inorganic Carbon (DIC) in the water: CO2, HCO3, H2CO3, CO32, which can be mutually transformed and mainly affected by the pH value of water
The result shows that this method has simple pretreatment, good selectivity and high sensitivity, which is appropriate for the determination of carbonate contents in cyanobacteria medium
The leacheate with high background conductance and cation exchange column of Optimix C18/SCX were used to produce negative chromatographic peak and baseline-resolved columns are chosen for further detection and determination
Summary
There are four forms of Dissolved Inorganic Carbon (DIC) in the water: CO2, HCO3-, H2CO3, CO32-, which can be mutually transformed and mainly affected by the pH value of water. At present, Liu et al (2006), Fischer (2002), XuChuan (2009) and Chen et al (2006) determined the carbonate utiling respectively these method of titrimetry, colorimetry, chromatography and enzymatic analysis. These methods can be applied in a certain range, but quantitative determination is more rapid and sensitive. The interference of matrix can be eliminated, which realizes the quantitative determination of carbonate contents in cyanobacteria medium. The result shows that this method has simple pretreatment, good selectivity and high sensitivity, which is appropriate for the determination of carbonate contents in cyanobacteria medium
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