Abstract
Simple, selective and sensitive spectrophotometric method has been developed for the determination of Azacitidine in pharmaceutical formulations and blood with MBTH (3-methyl-2-benzothiazolone hydrazone hydrochloride) reagent, at PH-4.0 which is extractable at 620 nm. Beer’s law is obeying in the concentration ranges 10 - 35 μg·ml-1 for formulations and 4 - 24 μg·ml-1 for blood sample. %R.S.D was found to be 0.0240%, 0.0610 and Recovery 99.82% 99.24% respectively. The method was completely validated and proven to be rugged. The interferences of the other ingredients and excipients were not observed. The repeatability and the performance of the proven method were conventional by point and interior proposition and through recovery studies.
Highlights
Azacitidine is a pyrimidine nucleoside analog of cytidine
Selective and sensitive spectrophotometric method has been developed for the determination of Azacitidine in pharmaceutical formulations and blood with MBTH (3-methyl-2-benzothiazolone hydrazone hydrochloride) reagent, at PH_4.0 which is extractable at 620 nm
An antitumor nucleoside approves by FDA for the action of myelodysplastic disorder [1]
Summary
Azacitidine is a pyrimidine nucleoside analog of cytidine. An antitumor nucleoside approves by FDA for the action of myelodysplastic disorder [1]. It is chemically 4-amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,2-di hydro-1,3,5-triazin-2-one (Figure 1) with molecular weight of 244.205 g/mol. It has verified activity against various solid tumors as well as leukemia [2]. The literature survey exposed that Azacitidine was determined by Liquid chromatography-mass spectrometry [5] [6], HPLC [7] [8], instrumental methods and spectrophotometric method using oxidative coupling reaction [9] [10] [11].
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