Abstract
A fully automated HPLC method was developed for the analysis of atrazine in water. This method used a high-performance immunoaffinity column for the extraction of atrazine and other triazines from samples, followed by separation of the refined compounds with an on-line reversed-phase column. This technique used only 250 μL of sample and required minimal sample pretreatment. Atrazine determinations by this method showed no significant interferences from the sample matrix, related triazines, or several common pesticides tested. Atrazine plus all of its major degradation products could be determined in 20 min, with a throughput of 10 min per injection
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