Determination of amino acid pairs sensitive to variants in human low-density lipoprotein receptor precursor by means of a random approach.

Abstract

In this data-based theoretical analysis, we use the random approach to analyse the amino acid pairs in human low-density lipoprotein receptor (LDL receptor) precursor in order to determine which amino acid pairs are more sensitive to 127 variants from missense mutant human LDL receptor. The rationale of this study is based on our hypothesis and findings that the harmful variants are more likely to occur at randomly unpredictable amino acid pairs, the unharmful variants are more likely to occur at randomly predictable amino acid pairs. This is because we argue that the randomly predictable amino acid pairs should not be deliberately evolved, whereas the randomly unpredictable amino acid pairs should be deliberately evolved with connection of protein function. The results show, for examples, 96.06% of 127 variants occur at randomly unpredictable amino acid pairs, which account for 80.44% of amino acid pairs in LDL receptor, and the chance of occurring of variant is about five times higher in randomly unpredictable amino acid pairs than in predictable pairs. Thus, the randomly unpredictable amino acid pairs are more sensitive to variants in human LDL receptor. The results also suggest that the human LDL receptor has the natural tendency to variants.