Abstract
The determination of serum biomarkers such as alpha-fetoprotein may be employed for early diagnosis of hepatocellular carcinoma. A miniature quartz crystal microbalance integrated with a polydimethylsiloxane microfluidic device was fabricated for on-chip determination of alpha-fetoprotein. Atomic force micrographs showed that anti-alpha fetoprotein was immobilized on the gold surface of the resonator using a cross-linking method. The integrated microfluidic device determined alpha-fetoprotein with a linear range from 0.3 to 3.74 µg/mL. By using phosphate buffer containing 200 µg/mL bovine serum albumin, 0.5 M NaCl, 500 µg/mL dextran, and 0.5% Tween 20, alpha-fetoprotein was determined in the presence of 75% human serum. Additionally, high temperature treatment allowed recovery of the resonator surface allowing limited reuse of the integrated microfluidic device.
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